Immunofluorescent analysis of SSEA-4 using anti-SSEA-4 monoclonal antibody (Product # MA1-021) shows staining on the cell surface of human H9 human embryonic stem cells, indicating pluripotency. SSEA-4 staining (green) and an overlay image of SSEA-4 with DAPI (blue) is shown. H9 human embryonic stem cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing SSEA-4 (Product # MA1-021) at a dilution of 1:40 overnight at 4 °C, washed with PBS and incubated with a FITC-conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification.
Immunofluorescent analysis of SSEA4 using anti-SSEA4 monoclonal antibody (Product # MA1-021) shows expression in human teratocarcinoma NCCIT cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing SSEA4 (Product # MA1-021), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product # 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
Supplier Page from Thermo Fisher Scientific for SSEA4 Antibody